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In glycolysis , glucose is split into two molecules. This process occurs in the cell's cytoplasm . The next stage of cellular respiration, the citric acid cycle, occurs in the matrix of eukaryotic cell mitochondria . In this stage, two ATP molecules along with high energy molecules (NADH and FADH 2 ) are produced. NADH and FADH 2 carry electrons on to the electron transport system. In the electron transport stage, ATP is produced by oxidative phosphorylation. In oxidative phosphorylation, enzymes oxidize nutrients resulting in the release of energy. This energy is use to convert ADP to ATP. Electron transport also occurs in mitochondria.